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# Mechanical strain exacerbates Pseudomonas infection in an organoid-based pneumonia-on-a-chip model
- URL: https://www.alveotalks.com/mechanical-strain-exacerbates-pseudomonas-infection-in-an-organoid-based-pneumonia-on-a-chip-model/
- Published: 2025-12-30T17:39:56.000Z
- Updated: 2026-02-22T14:23:11.000Z
- Author: Elena Lopez-Rodriguez
- Tags: The alveolar epithelial glycocalyx, #Open Access, #Publication

*Karen Hoffmann, Ulrike Behrendt, Peter Pennitz, Holger Kirsten, Jessica Pohl, *Elena Lopez-Rodriguez*, Chantal Weissfuss, Jens Kollmeier, Mario Tönnies, Sebastian Brill, Konrad Steinestel, Martin Witzenrath, Werner Wenzel, Christian Zobel, and Geraldine Nouailles. J Clin Invest. 2025 (In-Press Preview)* 

To access the full article, [click here](https://www.jci.org/articles/view/192454?ref=alveotalks.com): 

[![](https://www.alveotalks.com/content/images/2024/03/open-access.png)](https://www.jci.org/articles/view/192454?ref=alveotalks.com)

Pseudomonas aeruginosa ventilator-associated pneumonia (VAP) is one of the most frequent nosocomial infections in mechanically ventilated ICU patients, with mortality rates ranging from 24 to 76%, worsened by multi-drug resistance. Still, VAP pathophysiology remains unclear, partly due to limited model systems. Today, organotypic models, like the Emulate® alveolus chip, can replicate alveolar-capillary physiology, including air-liquid interface (ALI), vascular flow, and mechanical forces. Here, we advanced the alveolus-chip into a Pseudomonas pneumonia-on-a-chip (POC) model using human primary pulmonary microvascular endothelial cells (HPMVECs) and human alveolar epithelial cells of different origin.